Device

Part:BBa_J119386

Designed by: Julia Preziosi, Jon Trocosso   Group: Eckdahl Lab   (2015-06-09)


tCloneTetRed; Fusion Protein Reporter

This part was made from adding the TetA gene from part J119140 in front of the RFP gene in the tCloneRed part J119361. The Tet and RFP genes were combined, by deleting the "stop" sequence in the Tet gene and adding a linker sequence of proteins GGGS x4 to create a fusion protein. The resultant protein is a single protein with both the Tetracycline resistance protein and the Red fluorescent protein, combined with a linker chain. This part still contains tClone, and will not transcribe the reporter gene (Tet+Red) without the insertion of a riboswitch etc. between the BsaI sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1266
    Illegal NgoMIV site found at 1634
    Illegal NgoMIV site found at 1794
    Illegal AgeI site found at 2741
    Illegal AgeI site found at 2853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849
    Illegal BsaI.rc site found at 42


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Categories
Parameters
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